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Microbiology · Sterilization
Sample itemWhat does autoclaving use?
Original practice item, not a recalled exam question. Nothing here is recorded.
The answer and the reasoning
A. Pressurized steam
Autoclaving uses pressurized saturated steam to destroy microorganisms including spores when correct time and temperature are achieved.
Option by option
A. Pressurized steam (correct)
Correct: steam under pressure sterilizes.
B. Ultraviolet light only
UV has limited penetration.
C. Dry cold air
Cold air does not sterilize reliably.
D. Alcohol wipe only
Alcohol wipes do not reliably kill spores.
Source note: Original NextPrep sample item — pending expert review. This item is original practice material awaiting independent expert review, so confirm important facts in a standard textbook.