Enzymes: practice question

A Biochemistry question in the style of the NEET PG paper. Answer it first, then read why the right option is right and why each of the others is wrong.

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Biochemistry · Enzymes

Sample item

What does Vmax depend on when substrate is saturating?

Original practice item, not a recalled exam question. Nothing here is recorded.

The answer and the reasoning

B. Amount of active enzyme

At saturating substrate, reaction rate approaches Vmax, which is determined principally by the concentration and catalytic capacity of active enzyme.

Option by option

  • A. Temperature at one instant only

    Temperature can affect activity but does not define Vmax alone.

  • B. Amount of active enzyme (correct)

    Correct: more active enzyme raises maximal catalytic capacity.

  • C. Substrate affinity only

    Affinity is reflected by Km rather than Vmax.

  • D. Plasma albumin only

    Albumin is unrelated to most enzyme assays.

Source note: Original NextPrep sample item — pending expert review. This item is original practice material awaiting independent expert review, so confirm important facts in a standard textbook.

More Biochemistry questions

Questions about this Biochemistry question

What is the answer to this Biochemistry question?

The best answer is B, Amount of active enzyme. At saturating substrate, reaction rate approaches Vmax, which is determined principally by the concentration and catalytic capacity of active enzyme.

Why is "Temperature at one instant only" not the answer?

Temperature can affect activity but does not define Vmax alone.

Why is "Substrate affinity only" not the answer?

Affinity is reflected by Km rather than Vmax.

Why is "Plasma albumin only" not the answer?

Albumin is unrelated to most enzyme assays.

What concept does this question test?

It tests Enzymes in Biochemistry. The correct option holds because: Correct: more active enzyme raises maximal catalytic capacity.

How hard is this question?

We tag it as an application question: it asks you to use a fact on a patient or a finding. The tag is our own editorial judgement, not an official difficulty rating, so read the reason for every option, not only the answer.

How should I study Enzymes?

Build one table of enzyme deficiency, disease, inheritance and key finding, then revise it by spaced recall. Then use the flashcards and the other Biochemistry questions on this site to test what stuck.

Is this a real NEET PG question?

No. It is an original practice item (editorial status: sample, version 1). NextPrep does not publish recalled or leaked questions, and independent faculty review of this item has not been completed yet, so confirm important facts in a standard textbook.